Ordering re-targeted ClosTron plasmids from ATUM is very easy, but to ensure that there are no mistakes, please follow these instructions carefully:
Open the ATUM website
Click on the ‘Place an Order’ form
Complete the form as follows:
Sequence type: DNA
Sequence title (optional): Enter the name of your target, such as “Cdi-geneA-123s”
Upload sequence file...: DO NOT USE
Or Paste/type sequence...: Enter your 353 bp intron targeting region from the design tool results
Additional 5' sequence (optional): LEAVE BLANK
Additional 3' sequence (optional): LEAVE BLANK
Optimize sequence: Make as ordered
Select Order Preference: Regular gene synthesis (DNA-2-Go™)
Vector choice: Custom cloning
Other Requests: LEAVE BLANK
Notes: Enter “ClosTron” followed by your choice of ClosTron plasmid, such as “pMTL007C-E2”
Click ‘Add This Item To Cart’
Continue to enter payment and delivery details, and complete the order
If your name is not on the MTA between your institution and The University of Nottingham (see the legal stuff section) then when ordering please note the name of your group leader, or the name of whoever at your institution signed the MTA.