ClosTron.com is a resource for scientists and engineers, especially those who work on bacteria from the genus Clostridium. The site is provided and maintained by Nigel Minton, John Heap and the rest of the Clostridia Research Group (CRG) at The University of Nottingham. We established ClosTron.com to share some of our work with the research community. In particular, we want to make some methods and tools we have developed as accessible and easy to use as possible.
Everything you need to construct mutants in Clostridium using the ClosTron mutagenesis system. Includes background information, protocols and bioinformatics (the intron design tool).
Easily construct and modify Clostridium–E. coli shuttle plasmids in a standard way using our modular system. Automatically generate and download annotated plasmid sequences, then request the kit to get started (free for academics). We currently distribute 18 modules as 5 plasmids, from which 400 combinations can be made.
Information about ongoing Clostridium research in our group, which includes human and animal pathogens, strains which produce useful substances like biofuels, and even species with therapeutic potential.